Composite

Part:BBa_K3260004:Design

Designed by: Dominique WuDunn   Group: iGEM19_BrownStanfordPrinctn   (2019-08-29)


6x-His hG-CSF with TEV Cut Site & PpiB Solubility Factor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal AgeI site found at 342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 547
    Illegal BsaI.rc site found at 20
    Illegal SapI site found at 290


Design Notes

The coding sequence was optimized for E.coli using the IDT optimization tool. The standard "CAT" repeats for his-tag were diversified into both "CAT" and "CAC" on advice from our mentor.


Source

See basic parts for sources.

References